Bioptigen OCT systems utilize a narrow single-mode beam from a wide bandwidth light source to probe the structure of a sample. A single line image of the internal structure of a sample is commonly known as an A-scan, in analogy to ultrasound. By scanning the single-mode beam laterally in on dimension, a two-dimensional B-scan is acquired. A B-scan provides an informative depth-resolved image along a slice without ever making a cut. A series of B-scans can be acquired to develop a three-dimensional image suitable for visualizing internal structures, layers, and volumes with high resolution in all three dimensions.
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